DNA synthesis inhibiting factors of muscle-cornin and cornea-cornin
نویسندگان
چکیده
منابع مشابه
Antimitotic action of cornin as a biologically active polypeptide. I. Biochemical properties of cornin.
We succeeded in the extraction of a substance from beef cornea and rabbit muscle, that markedly inhibits mitosis of sea urchin eggs. The substance extracted from beef cornea is nondialysable and it can be separated into three fractions by DEAE-cellulose column. Although everyone of these fractions has an antimitotic action, that of fractions II and III is especially marked. These fractions are ...
متن کاملStudies on the cornin extracted from bovine liver. II. Inhibitory effect of the cornin on DNA synthesis and cell growth of L cells cultured in suspension.
Gornin was extracted from bovine liver. The effects of cornin on DNA synthesis were compared with its effects on cell growth using L cells growing in suspension. As the first step of this experiment, a simple method of suspension culture was established with a new modification of YLE medium. Both effects of cornin paralleled with dosage. And the properties of the inhibitory factor of DNA synthe...
متن کاملAntimitotic action of cornin as a biologically active polypeptide. II. Physiological effects of cornin on dividing cell.
Both the cornea and muscle cornins have no effect at all on oxygen uptake of tissue, and likewise they catalase affect Qctivity not in any way. The corneacornin has an effect to reduce P/O ratio to about one half, but the muscle cornin does not show such an effect. Both comins decrease thc incorporation of P into nucleic acid fraction and DNA synthesis. In the ultracentrifugal analysis of nucle...
متن کاملStudies on the cornin extracted from bovine liver. I. Purification of the cornin and its physico-chemical properties.
A factor, cornin, inhibiting the growth of L cells cultured in monolayer was extracted from bovine liver with boiling water and was partially purified by gel filtration with Sephadex G-200. The factor was (1) precipitable with ethanol at the concentration between 70% and 90%, (2) impermeable through dializing memo brane, (3) eluted as the last peak at the gel filtration and (4) containing prote...
متن کاملCardioprotection against experimental myocardial ischemic injury using cornin
Phosphorylated-cyclic adenosine monophosphate response element-binding protein (Phospho-CREB) has an important role in the pathogenesis of myocardial ischemia. We isolated the iridoid glycoside cornin from the fruit of Verbena officinalis L, investigated its effects against myocardial ischemia and reperfusion (I/R) injury in vivo, and elucidated its potential mechanism in vitro. Effects of corn...
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ژورنال
عنوان ژورنال: Okayama Igakkai Zasshi (Journal of Okayama Medical Association)
سال: 1972
ISSN: 0030-1558,1882-4528
DOI: 10.4044/joma1947.84.5-6_119